蜂毒素对骨肉瘤UMR-106细胞裸鼠移植瘤血管生成影响.docVIP

蜂毒素对骨肉瘤UMR-106细胞裸鼠移植瘤血管生成影响摘 要：目的：本研究拟探讨蜂毒素抗骨肉瘤UMR-106细胞裸鼠胫骨移植瘤的作用及其对肿瘤血管生成的影响。方法：建立骨肉瘤UMR-106细胞裸鼠胫骨移植瘤模型，成瘤后随机分为两组：生理盐水组、蜂毒素治疗组。瘤内注射给药治疗10天，计算治疗组肿瘤的体积抑制率和重量抑制率；采用免疫组化CD105观察移植瘤的微血管密度(MVD)；免疫组化和Western blot检测瘤组织VEGF、HIF-1α蛋白表达。结果：蜂毒素组对骨肉瘤的重量抑制率为38.92%，体积抑制率为43.04%(P0.05)。免疫组化及Western blot检测结果显示蜂毒素组CD105蛋白标记的MVD、VEGF、HIF-1α蛋白表达水平均明显低于生理盐水组(P0.01)。结论：蜂毒素能明显抑制骨肉瘤UMR-106细胞裸鼠胫骨移植瘤的血管生成，从而抑制肿瘤生长。与蜂毒素能够下调瘤组织VEGF、HIF-1α蛋白表达有关。 关键词：蜂毒素；骨肉瘤；血管生成；裸小鼠 中图分类号：R-33 文献标识码：A 文章编号：1673-7717(2008)03-0522-03 Effects of Melittin on Angiogenesis of OsteosarcomaCell UMR-106 Xenograft in Nude Mice GAO Qi-long1，LI Yu-mei1，CHEN Yong-qiang1，LI Bai2，LING Chang-quan2. (1. Orthopedics Department，Shanghai Hospital of Traditional Chinese Medicine Affiliated Shanghai University of Chinese Medicine， Shanghai 200071，China；2.Institute of Integrated Traditional Chinese and Western Medicine，Changhai Hospital Affiliated Second Military Medical University，Shanghai 200433，China) Abstract：Objective：To investigate the Effects of Melittin on the Growth and Angiogenesis of OsteosarcomaCell UMR-106 Xenografts in nude mice. Methods：Orthotopically transplanted tumor of osteosarcoma was established in nude mice. The mice were randomly divided into two groups：NS-treated group and melittin group(320μg/kg).The drug was injected intratumorally for 10 days. The tumor volume and weight inhibition rates were calculated. The MVD changes of tumor tissues were detected by CD105 staining in vivo，and the expressions of VEGF，HIF-1α proteins were determined by immunohistochemical and western blot method. Results：The tumor volume inhibition rates was 43.04%，and the weight inhibition rates was 38.92%. The MVD and the expressions of VEGF，HIF-1α proteins of milittin group were fewer than that of NS-treated group (P0.01). Conclusion：Melittin could inhibit the growth of osteosarcoma UMR-106 xenografts in nude mice. The down-regulation of VEGF，HIF-1α expression and the inhibition of VCD might play a key r