Logitp四参数拟合科研数据处理方法.doc

科研数据处理方法——四参数拟合 科研工作者必须要掌握的技能。然而，由于数据形式的多种多样，伴随着方法也是层出不穷。对于免疫检测的数据处理，常常会用到四参数拟合，下面介绍其具体的数据处理方法。 酶标仪或者紫外分光光度计 免疫分析数据 首先在Excel中整理好数据，一部分是做标准曲线的数据，另一部分是待求的数据。 打开ELISA Calc软件，先在Excel中复制数据，再在软件中点击“粘贴”。 选择回归/拟合模型，选中“四参数”，点击“回归/拟合按钮”。 弹出的窗口中显示了曲线，可以截图下来。别忘了X和Y分别表示的什么。这是标准品提供一个方程。样品有反应值，要计算X值，点击“由Y计算X”。 复制Excel中的样品的反应值，点击软件的“粘贴”按钮，即可自动计算出对应的X值，也即是样品浓度。 方程拟合的好坏，还可以查看方程来检验，主要看r^2的值，越接近1，拟合效果越好。 本方法不限于做四参数拟合，其它拟合方法相似。 of vision. 3, build, fitting in with the urban master plan in Yibin city rapid rail transit long term network, as well as the vision of Yibin city, and future development of rail transit network. 4, Yibin city, preliminary rail traffic levels service levels, system model, and technical standards. 5, to ensure that network can be implemented. 6, stable conditions for planning and supporting of Yibin city track traffic construction and formation of rail transit industry, provide conditions for the start-up and development of rail transit in Yibin city. 1.7 technical course technology is the projects research program and research ideas. Technology reflects the various stages in the process, logical relations between various levels, research, techniques and phases. Network planning process the whole process can be divided into four parts, namely, background research, network architecture research, planning and implementation of research and programming interfaces. Figure 1.7-1 Yibin city rail transit network planning technology roadmap planning interface is mainly responsible for network planning interface with follow-up planning tasks. Network planning Urban planning system in a transitional position, online network planning is completed, will be carried out as soon as possible the following planning projects: 1, detailed control of the land of rapid rail transit network planning; 2, the partitioning for network planning; 3, joint planning of rail transport and urban transport in other ways; Detailed technical courses see below. Figure 1.7-2 General technolog