双眼形觉剥夺后成年大鼠视皮层可塑性再激活模型建立.docVIP

双眼形觉剥夺后成年大鼠视皮层可塑性再激活模型建立 余涛，阴正勤，翁传煌（第三军医大学西南眼科医院，重庆 400038） 基金项目：国家自然科学基金资助项目30672280），863计划项目（2007AA04Z324） Supported by the National Natural Science Foundation of China 30672280) and the National High Technology Research and Development Program of China (863 program)（2007AA04Z324） 作者简介：余涛，女，（1974-），400038，重庆市人，博士，主治医师。主要从事视皮层可塑性及弱视发病机制的研究。电话：(023联系电话E-mail: yt423@ 通讯作者：阴正勤，电话：(023E-mail: zqyin@ 提要：目的 建立成年大鼠双眼形觉剥夺后视皮层可塑性再激活模型。方法 采用视觉诱发电位(visual evoked potentials，VEPs) 记录方法验证Long-Evans大鼠视皮层可塑性关键期结束的时间，然后采用成年大鼠进行双眼形觉剥夺（binocular form deprivation, BFD)，最后选择短期(3天)单眼剥夺（monocular deprivation, MD)就能引起眼优势移动的BFD时间。结果 视皮层可塑性关键期在 大鼠出生后5周龄(postnatal weeks，PW)后结束；从PW6开始，MD就不能造成年大鼠视皮层眼优势的移动；BFD14天后的成年大鼠视皮层眼优势可以在短期MD后发生移动。结论 BFD可成功激活成年大鼠视皮层可塑性，此成年大鼠视皮层可塑性再激活模型的建立为成年弱视治疗的进一步研究奠定了基础。 关键词：成年；弱视；双眼形觉剥夺；眼优势；视皮层；可塑性 A model of binocular form deprivation reactivating ocular dominance plasticity in adult visual cortex YU Tao, YIN Zheng-qin, WENG Chuan-huang (Southwest Eye Hospital, Third Military Medical University，Chongqing, 400038, China) Abstract: Objective To build the animal model whose visual cortex plasticity was reactivated by long time’s binocular form deprivation (BFD) from adulthood. Methods Visual evoked potentials (VEPs) were recorded in the pigmented Long–Evans rats aged from postnatal week (PW) 3 to PW7 and recorded again after 3d, 5d or 7d’s monocular deprivation (MD) in the right eyes. Rats of PW7 were treated with BFD for 7d, 10d or 14d, and then left eyes were opened for 3d, 5d or 7d befor the next VEPs recording. Results Brief (3d) MD decreased C/I ratio (contralateral VEPs amplitude/ ipsilateral VEPs amplitude to the occluded eye) in rats from PW3, PW4 and PW5 but not in those from PW6 and PW7, confirming the absence of rapid OD plasticity in adults. However, when brief MD was preceded by 14 d of BFD, we observed a significant decreasing in the C/I ratio in rats from P7W, demonstrating the reactivation of rapid OD