不同生态条件下玉米单倍体自然加倍率及除草剂加倍效果.PDF

安徽农业大学学报, 2017, 44(1): 114-118 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x010 网络出版时间：2017/2/8 9:43:00 [URL] /kcms/detail/34.1162.S0943.020.html 不同生态条件下玉米单倍体自然加倍率及除草剂加倍效果 * 程 江，郑常祥，潘敏娜，陈柔屹 (贵州省旱粮研究所，贵阳 550006) 摘 要：单倍体加倍率低成为玉米单倍体育种的限制性因素。为了提高玉米单倍体的加倍率，研究两种生态条 件下单倍体的自然加倍率，比较甲基胺草磷（APM ）和氟乐灵两种除草剂在不同浓度和处理方法下玉米单倍体的 加倍效率。结果表明，海南试验点的单倍体自然加倍率要高于贵州贵阳试点。应用除草剂可以明显提高雅玉 889 和京糯208 单倍体的加倍率，基础材料基因型对自然加倍率和除草剂加倍率都具有显著影响。APM 和氟乐灵均是 在浓度为80 μmol·L-1 处理单倍体加倍率最高，APM 处理的单倍体加倍率要高于氟乐灵，采用滴心法和浸种法处理 的单倍体加倍率无明显差异。综上，如采用自然加倍，应在海南试验点种植单倍体；如采用除草剂加倍，应使用浓 度为80 μmol·L-1 的APM ，采用滴心法或浸种法对雅玉889 和京糯208 单倍体加倍。 关键词：玉米；单倍体；自然加倍；除草剂 中图分类号：S513 文献标识码：A 文章编号：1672352X (2017)01011405 Effects of the ecological condition and herbicide on chromosome doubling of haploid maize CHENG Jiang, ZHENG Changxiang, PAN Minna, CHEN Rouyi (Guizhou Institute of Upland Food Crops, Guiyang 550006) Abstract: A low chromosome doubling rate of haploids becomes the limiting factor in maize haploid breed- ing at present. The purpose of this experiment was to improve the doubling rate of maize haploid in two ecological systems and through the herbicide application. Two herbicides, amiprophos-methyl (APM) and trifluralin at dif- ferent concentrations with different methods were tested. The 2-year experimental result showed that the natural doubling rate of maize haploids grown in Jiusuo, Hainan Province was much higher than that in Guiyang, Guizhou Province. The doubling rates of haploid Yayu889 and Jingnuo208 were significantly improved with the application of herbicides. The doubling rate was also s