abnormal shifts in raman spectra of deuterated cytidine and 6-azacytidine拉曼光谱的变化异常氘胞嘧啶核苷和6-azacytidine.pdfVIP

Spectroscopy 24 (2010) 191–195 191 DOI 10.3233/SPE-2010-0461 IOS Press Abnormal shifts in Raman spectra of deuterated cytidine and 6-azacytidine S. Garasevych, M. Iakhnenko ∗, O. Slobodyanyuk and I. Vaskivskyi Department of Physics, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine Abstract. It was revealed that some peaks in Raman spectra of cytidine, 6-azacytidine (6-azaC), and cytosine dissolved in D O are shifted to high frequencies in respect to their positions in spectra of H O solution. Such “blue shifts” occur due 2 2 to deuteration of the nucleoside molecule itself but not due to effect of deuterated solvent. This conclusion is deduced from observation of blue (abnormal) shift in Raman spectra of cytidine and 6-azaC microcrystals recrystallized from D2 O solution. Both normal and abnormal shifts close to the experimentally observed were obtained in the calculated spectra of 6-azaC and cytidine. We assume that abnormal shifts may be caused by substitution of intramolecular H-bonds with D-bonds. Keywords: Raman spectra, cytidine, 6-azacytidine, blue shift, H-bonds, deuteration 1. Introduction The cytidine is well-known canonical nucleoside and anomalous nucleoside 6-azacytidine (6-azaC) is a structural analogue thereof with N atom replacing of C–H group at the 6th position of pirimidine ring ([5] and references therein). The 6-azaC is an antimetabolite of nucleic acid exchange and considered as a prospective pharmaceutical component with a wide spectrum of therapeutic effects [1]. The 6-azaC is usually available in microcrystalline form but its valuable biological activity appears in solutions and not in crystalline state. Raman spectra of 6-azaC were measured for the first time both in microcrystalline form and in different parti