absence of telomerase activity in malignant bone tumors and soft-tissue sarcomas缺乏端粒酶活性在恶性骨肿瘤和软组织肉瘤.pdfVIP

Sarcoma (2002) 6, 43–46 ORIGINAL ARTICLE Absence of telomerase activity in malignant bone tumors and soft-tissue sarcomas 1 2 3 4 NINA K. LAUER , SANDRA M. MAIER , MARTIN OBERRINGER , MICHAEL SCHULTE , 5 3 6 WOLF MUTSCHLER , RAINER G. HANSELMANN , MANFRED SCHARTL , STEFAN J. SCHERER6 REINER J. WIRBEL7 1Department of Reconstructive Surgery, University of Saarland, D-66421 Homburg/Saar, Germany, 2Department of Human Genetics, University of Saarland, Geb. 68, D-66421 Homburg/Saar, Germany, 3Department of Experimental Physics, University of Saarland, Geb. 22, 68111 Saarbrücken, Germany, 4Department of Trauma,Hand and Reconstructive Surgery, University of Ulm, 89070 Ulm, Germany, Department of Experimental Physics, University of Saarland, Geb. 22, 68111 Saarbrücken, Germany, 5Department of Surgery, Ludwig Maximillian-University of Munich, Klinikum Innenstadt, 80336 Munich, Germany, 6Department of Physiological Chemistry I, Biocenter, University of Wuerzburg, 97074 Wuerzburg, Germany 7Department of Surgery, Elisabeth-Hospital, 56564 Neuwied, Germany Abstract Purpose: Telomerase activity appears to play a crucial role in the development of many tumors. More than 80% of all malig- nant human tumors show an increased telomerase activity. However, conflicting results have been reported about telomerase activity in sarcomas. The aim of the study was to obtain more information about telomerase activity in sarcomas based on a large number of cases. Methods: Telomerase activity was measured in 69 different tumor samples (33 malignant bone tumors and 36 soft tissue sarcomas). Tumor samples were obtained intraoperatively and frozen immediately in liquid nitrogen. Telomerase activity was detected by the telomeric repeat ampli