bifidobacterium animalis ssp. lactis 420 protects against indomethacin-induced gastric permeability in rats双歧杆菌animalis ssp。.pdfVIP

Hindawi Publishing Corporation Gastroenterology Research and Practice Volume 2012, Article ID 615051, 9 pages doi:10.1155/2012/615051 Research Article Bifidobacterium animalis ssp. lactis 420 Protects against Indomethacin-Induced Gastric Permeability in Rats Anna Lyra,1 Markku Saarinen,1 Heli Putaala,1 Kaisa Olli,1 Sampo J. Lahtinen,1 Arthur C. Ouwehand,1 Mari Madetoja,2 and Kirsti Tiihonen1 1 DuPont Nutrition and Health, Kantvik Active Nutrition, Sokeritehtaantie 20, 02460 Kantvik, Finland 2 Toxis, SBW Corp. Ltd., Lemminkaisenkatu 14-18 C, 20520 Turku, Finland ¨ Correspondence should be addressed to Anna Lyra, anna.lyra@ Received 30 December 2011; Revised 30 March 2012; Accepted 2 May 2012 Academic Editor: Emidio Scarpellini Copyright © 2012 Anna Lyra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Gastrointestinal (GI) adverse effects such as erosion and increased permeability are common during the use of nonsteroidal anti-inflammatory drugs (NSAIDs). Our objective was to assess whether Bifidobacterium animalis ssp. lactis 420 protects against NSAID-induced GI side effects in a rat model. A total of 120 male Wistar rats were allocated into groups designated as control, NSAID, and probiotic. The NSAID and probiotic groups were challenged with indomethacin (10 mg/kg−1; single dose). The probiotic group was also supplemented daily with 1010 CFU of B. lactis 420 for seven days prior to the indomethacin administration. The control group rats received no indomethacin or probiotic. The permeability of the rat intestine