bioactive markers based pharmacokinetic evaluation of extracts of a traditional medicinal plant, piper sarmentosum提取物的生物活性标记基于药代动力学评价传统的药用植物,派珀sarmentosum.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 980760, 7 pages doi:10.1093/ecam/nep143 Original Article Bioactive Markers Based Pharmacokinetic Evaluation of Extracts of a Traditional Medicinal Plant, Piper sarmentosum Khalid Hussain, Zhari Ismail, Amirin Sadikun, and Pazillah Ibrahim Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia Correspondence should be addressed to Khalid Hussain, hussain 761@ Received 4 February 2009; Accepted 25 August 2009 Copyright © 2011 Khalid Hussain et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In vitro assays are economical and easy to perform but to establish relevance of their results to real clinical outcome in animals or human, pharmacokinetics is prerequisite. Despite various in vitro pharmacological activities of extracts of Piper sarmentosum, there is no report of pharmacokinetics. Therefore, the present study aimed to evaluate ethanol extract of fruit of the plant in dose of 500 mg kg−1 orally for pharmacokinetics. Sprague-Dawley rats were randomly divided into groups 1, 2, and 3 (each n = 6) to study absorption, distribution and excretion, respectively. High performance liquid chromatography (HPLC) with ultraviolet detection was applied to quantify pellitorine, sarmentine and sarmentosine in plasma, tissues, feces and urine to calculate pharmacokinetic parameters. Pellitorine exhibited maximum plasma concentration (Cmax ) 34.77 ng mL−1 ± 1.040, time to achieve Cmax (Tmax ) 8 h, mean resident time (MRT) 26.00 ± 0.149