bacillus amyloliquefaciens g1 a potential antagonistic bacterium against eel-pathogenic aeromonas hydrophila芽孢杆菌amyloliquefaciens g1潜在的拮抗细菌对eel-pathogenic气单胞菌属hydrophila.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 824104, 7 pages doi:10.1155/2011/824104 Research Article Bacillus amyloliquefaciens G1: A Potential Antagonistic Bacterium against Eel-Pathogenic Aeromonas hydrophila Haipeng Cao,1 Shan He,2 Ruopeng Wei,3 Marek Diong,1 and Liqun Lu1 1 Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of Ministry of Education, Aquatic Pathogen Collection Center of Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China 2 Education Department, Shanghai Normal University, Shanghai 200235, China 3 Technology Department, Shanxi Veterinary Drug and Feed Engineering Technology Research Center, Yuncheng 044000, China Correspondence should be addressed to Liqun Lu, caohaipen Received 24 December 2010; Accepted 16 May 2011 Copyright © 2011 Haipeng Cao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Recent studies have revealed that the use of probiotics is an alternative to control marine aeromonas. However, few probiotics are available against Aeromonas hydrophila infections in eels. In the present study, a potential antagonistic strain G1 against the eel-pathogenic A. hydrophila was isolated from sediment underlying brackish water. Its extracellular products with antibacterial activities were shown to be stable under wide range of pH, temperature, and proteinase K. It was initially identified as Bacillus amyloliquefaciens using API identification kits and confirmed to be B. amyloliquefaciens strain (GenBank accession number DQ422953) by phylogenetic analysis. In a