bimodal porous scaffolds by sequential electrospinning of poly(glycolic acid) with sucrose particles双峰多孔支架的连续电纺聚乙醇酸)与蔗糖的粒子.pdfVIP

Hindawi Publishing Corporation International Journal of Polymer Science Volume 2010, Article ID 436178, 9 pages doi:10.1155/2010/436178 Research Article Bimodal Porous Scaffolds by Sequential Electrospinning of Poly(glycolic acid) with Sucrose Particles B. Wulkersdorfer,1 K. K. Kao,1, 2 V. G. Agopian,1, 2 A. Ahn,1 J. C. Dunn,2, 3 B. M. Wu,2, 3 and M. Stelzner1, 2 1 Department of Surgery, VA Greater Los Angeles Health Care System, Los Angeles, CA 90073, USA 2 Department of Surgery, University of California at Los Angeles, Los Angeles, CA 90095, USA 3 Department of Bioengineering, University of California at Los Angeles, Los Angeles, CA 90095, USA Correspondence should be addressed to M. Stelzner, matthias.stelzner@ Received 20 November 2009; Revised 20 January 2010; Accepted 20 January 2010 Academic Editor: Shanfeng Wang Copyright © 2010 B. Wulkersdorfer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Electrospinning is a method to produce fine, biopolymer mesh with a three-dimensional architecture that mimics native extra- cellular matrix. Due to the small fiber diameter created in this process, conventional electrospun scaffolds have pore sizes smaller than the diameter of most cells. These scaffolds have limited application in tissue engineering due to poor cell penetration. We developed a hybrid electrospinning/particulate leaching technique to create scaffolds with increased porosity and improved cellular ingrowth. Poly(glycolic acid) (PGA) and a sucrose-ethanol suspension were electrospun in equal, alternating sequences at intervals of one, two, and ten minutes each. The scaff