cardiovascular activity of labdane diterpenes from andrographis paniculata in isolated rat hearts心血管活动labdane二萜穿心莲香的孤立的老鼠的心脏.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2012, Article ID 876458, 5 pages doi:10.1155/2012/876458 Research Article Cardiovascular Activity of Labdane Diterpenes from Andrographis paniculata in Isolated Rat Hearts Khalijah Awang,1 Nor Hayati Abdullah,2 A. Hamid A. Hadi,1 and Yew Su Fong3 1 Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 2 Medicinal Plants Division, Forest Research Institute of Malaysia (FRIM), Kepong, 52109 Kuala Lumpur, Malaysia 3 Department of Pharmacy, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia Correspondence should be addressed to Khalijah Awang, khalijah@.my Received 29 July 2011; Revised 20 January 2012; Accepted 21 January 2012 Academic Editor: Masa-Aki Shibata Copyright © 2012 Khalijah Awang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The dichloromethane (DCM) extract of Andrographis paniculata Nees was tested for cardiovascular activity. The extract significantly reduced coronary perfusion pressure by up to 24.5 ± 3.0 mm Hg at a 3 mg dose and also reduced heart rate by up to 49.5 ± 11.4 beats/minute at this dose. Five labdane diterpenes, 14-deoxy-12-hydroxyandrographolide (1), 14-deoxy-11,12- didehydroandrographolide (2), 14-deoxyandrographolide (3), andrographolide (4), and neoandrographolide (5), were isolated from the aerial parts of this medicinal plant. Bioassay-guided studies using animal model showed that compounds, (2) and (3) were responsible for the coronary vasodilatation. T