cationic polybutyl cyanoacrylate nanoparticles for dna delivery阳离子polybutyl cyanoacrylate纳米粒子进行dna交付.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2009, Article ID 149254, 9 pages doi:10.1155/2009/149254 Research Article Cationic Polybutyl Cyanoacrylate Nanoparticles for DNA Delivery Jinghua Duan,1 Yangde Zhang,1 Wei Chen,1 Chengrong Shen,1 Mingmei Liao,1 Yifeng Pan,1 Jiwei Wang,1 Xingming Deng,2, 3 and Jinfeng Zhao1 1 Key Laboratory of Nanobiological Technology, Ministry of Health National Hepatobiliary and Enteric Surgery Research Center, Central South University, Changsha, Hunan 410008, China 2 Shands Cancer Center, University of Florida, Gainesville, FL 32610-0232, USA 3 Department of Medicine, University of Florida, Gainesville, FL 32610-0232, USA Correspondence should be addressed to Jinfeng Zhao, zhaojinfeng@ Received 4 July 2008; Revised 3 December 2008; Accepted 25 December 2008 Recommended by Hicham Fenniri To enhance the intracellular delivery potential of plasmid DNA using nonviral vectors, we used polybutyl cyanoacrylate (PBCA) and chitosan to prepare PBCA nanoparticles (NPs) by emulsion polymerization and prepared NP/DNA complexes through the complex coacervation of nanoparticles with the DNA. The object of our work is to evaluate the characterization and transfection efficiency of PBCA-NPs. The NPs have a zeta potential of 25.53 mV at pH 7.4 and size about 200 nm. Electrophoretic analysis suggested that the NPs with positive charges could protect the DNA from nuclease degradation and cell viability assay showed that the NPs exhibit a low cytotoxicity to human hepatocellular carcinoma (HepG2) cells. Qualitative and quantitative analysis of transfection in HepG2 cells by the nanoparticles carrying plasmid DNA encoding for enhanced green fluorescent protein (EGFP- N1) was done by digital fluorescence imaging microsc