repair of dna alkylation damage by the escherichia coli adaptive response protein alkb as studied by esi-tof mass spectrometry大肠杆菌修复dna的烷基化的适应性反应蛋白质alkb esi-tof质谱研究了.pdf

SAGE-Hindawi Access to Research Journal of Nucleic Acids Volume 2010, Article ID 369434, 9 pages doi:10.4061/2010/369434 Research Article Repair of DNA Alkylation Damage by the Escherichia coli Adaptive Response Protein AlkB as Studied by ESI-TOF Mass Spectrometry Deyu Li,1, 2, 3 James C. Delaney,1, 2, 3, 4 Charlotte M. Page,1, 2, 3, 5 Alvin S. Chen,1, 2, 3 Cintyu Wong,2, 3, 6, 7, 8 Catherine L. Drennan,2, 3, 6, 7 and John M. Essigmann1, 2, 3 1 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 2 Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 3 Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 4 Visterra Inc., Cambridge, MA 02142, USA 5 Department of Chemistry, Yale University, New Haven, CT 06520, USA 6 Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 7 Howard Hughes Medical Institute, USA 8 Johnson Johnson Pharmaceutical Research Development, 930 Route 202 South, Raritan, NJ 08869, USA Correspondence should be addressed to John M. Essigmann, jessig@ Received 5 August 2010; Accepted 15 September 2010 Academic Editor: Ashis Basu Copyright © 2010 Deyu Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. DNA alkylation can cause mutations, epigenetic changes, and even cell death. All living organisms have evolved enzymatic and non-enzymatic strategies for repairing such alkylation damage. AlkB, one of the Escherichia coli adaptive response proteins, uses an α-ketoglutarate/Fe(I