robust gene expression index强大的基因表达指数.pdf

Hindawi Publishing Corporation Mathematical Problems in Engineering Volume 2012, Article ID 182758, 12 pages doi:10.1155/2012/182758 Research Article Robust Gene Expression Index ˇ Vilda Purutc¸uoglu Department of Statistics, Middle East Technical University, 06531 Ankara, Turkey Correspondence should be addressed to Vilda Purutc¸uoglu, vpurutcu@.tr ˇ Received 10 October 2011; Accepted 22 October 2011 Academic Editor: Gerhard-Wilhelm Weber Copyright q 2012 Vilda Purutc¸uoglu. This is an open access article distributed under the Creative ˇ Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The frequentist gene expression index FGX was recently developed to measure expression on Affymetrix oligonucleotide DNA arrays. In this study, we extend FGX to cover nonnormal log expressions, specifically long-tailed symmetric densities and call our new index as robust gene expression index RGX. In estimation, we implement the modified maximum likelihood method to unravel the elusive solutions of likelihood equations and utilize the Fisher information matrix for covariance terms. From the analysis via the bench-mark datasets and simulated data, it is shown that RGX has promising results and mostly outperforms FGX in terms of relative efficiency of the estimated signals, in particular, when the data are nonnormal. 1. Introduction Microarray technology enables the measurement of RNA transcripted DNA expression levels. For this purpose, it uses different kinds of optical techniques, which quantify the colour in