role of histamine in the inhibitory effects of phycocyanin in experimental models of allergic inflammatory response组胺在藻青蛋白的抑制作用实验模型的过敏性炎症反应.pdf

Research Commmunication Mediators of Inflammation, 11, 81–85 (2002) IT has recently been reported that phycocyanin, a Role of histamine in the inhibitory biliprotein found in the blue– green microalgae Spir- ulina, exerts anti-inflammatory effects in some ani- effects of phycocyanin in mal models of inflammation. Taking into account experimental models of allergic these findings, we decided to elucidate whether phycocyanin might exert also inhibitory effects in the inflammatory response induced allergic inflammatory response and on hista- mine release from isolated rat mast cells. In in vivo experiments, phycocyanin (100, 200 and 300 mg/kg post-orally (p.o.)) was administered 1 h before the D. Remirez, N. Ledon´ and R. Gonzalez´ CA challenge with 1 m g of ovalbumin (OA) in the ear of mice previously sensitized with OA. One hour later, myeloperoxidase activity and ear edema were Ozone Research Center, National Center for assessed. Phycocyanin significantly reduced both Scientific Research, Post Box 6412, Havana, Cuba parameters. In separate experiments, phycocyanin (100 and 200 mg/kg p.o.) also reduced the blue spot area induced by intradermal injections of histamine, and the histamine releaser compound 48/80 in rat CACorresponding Author skin. In concordance with the former results, phyco- Fax: +53 7 210233 cyanin also significantly reduced histamine release E-mail: ozono@infomed.sld.cu induced by compound 48/80 from isolated peritoneal rat mast cells. The inhibitory effects of phycocyanin were dose dependent. Taken together, our results suggest that inhibition of allergic inflammatory response by phycocyanin is mediated, at least in part, by inhibition of histamine release from mast cells.