secretion of interleukin-8 by human-derived cell lines infected with mycobacterium bovis分泌的interleukin-8人为细胞系牛结核分枝杆菌感染.pdf

Short Communication Mediators of Inflammation, 13(1), 45/49 (February 2004) Background : The variable efficacy of bacillus Calm- Secretion of interleukin-8 by ´ ette-Guerin (Mycobacterium bovis BCG) in protecting humans against tuberculosis has prompted a search human-derived cell lines infected for the mechanisms through which BCG induces with Mycobacterium bovis chemokines. In this study, our experiments were designed to determine the role of the transcription factor nuclear factor-kB (NF-kB) and intracellular calcium in the production of interleukin (IL)-8, a Patricia Me´ndez-SamperioCA, Janet Palma-Barrios, main chemotactic factor, by human-derived mono- Abraham Va´zquez-Herna´ndez and Elizabeth cytic cell line U937 and by a human epithelial HEp-2 Garcıa-Martınez ´ ´ cell line infected with M. bovis BCG. Methods : The concentrations of IL-8 in culture super- natants of U937 cells or HEp-2 cells infected with M. bovis BCG were determined by enzyme-linked Departamento de Inmunologıa, Escuela Nacional de ´ immunosorbent assay. We used sulfasalazine and Ciencias Biolo´ gicas, IPN, Carpio y Plan de Ayala, curcumin, which are well-described inhibitors of Me´xico, D.F. 11340 Me´xico NF-kB activity, and we used ethylenediamine tetra- acetic acid to deplete extracellular Ca2 or used the cell-permeable agent 1,2-bis (2-aminophenoxy) eth- ane-N ,N ,N ?,N ?-tetraacetic acid tetra (acetoxymethyl) CACo