secretory leukoprotease inhibitor a native antimicrobial protein in the innate immune response in a rat model of s. aureus keratitis分泌leukoprotease抑制剂原生抗菌蛋白的先天免疫反应在老鼠模型中金黄色葡萄球菌角膜炎.pdf

Hindawi Publishing Corporation Journal of Ophthalmology Volume 2009, Article ID 259393, 5 pages doi:10.1155/2009/259393 Research Article Secretory Leukoprotease Inhibitor: A Native Antimicrobial Protein in the Innate Immune Response in a Rat Model of S. aureus Keratitis Victor E. Reviglio,1, 2 Andres Grenat,1 Federico Pegoraro,2 Ruben H. Sambuelli,1 Tayyib Rana,3 and Irene C. Kuo4 1 Cornea and Anterior Segment Research, Pathology Department, School of Medicine, Catholic University of Cordoba, Cordoba, Argentina 2 Ophthalmology Service, Cornea Department, Cordoba Hospital, Cordoba, Argentina 3 Cornea and Anterior Segment Department, Northern Virginia Eye Institute, Virginia, USA 4 Cornea Division, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA Correspondence should be addressed to Victor E. Reviglio, victor-reviglio@ Received 23 June 2009; Accepted 21 July 2009 Recommended by Edward Manche Purpose. To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity in the innate immune reaction in a rat model of Staphylococcus aureus keratitis. Methods. Forty female Lewis rats were divided into 2 groups: the infectious keratitis and the epithelial defect groups. Eyes were processed for immunohistochemical studies for SLPI, interleukin-1, interleukin-6, tumor necrosis factor-alpha, and matrix metalloproteinase-8. Results. Immunohistochemical studies confirmed high levels of SLPI, IL-1, IL-6, TNF-α, and MMP-8 expression in eyes with S. aureus keratitis and with epithelial defects, in contrast to undetectable SLPI expression in the normal control corneas. Conclusions. To our knowledge, this paper is the first to demonstr