short-term exposure to tobacco toxins alters expression of multiple proliferation gene markers in primary human bronchial epithelial cell cultures短期接触烟草毒素改变多个增殖基因标记的表达主要人类支气管上皮细胞培养.pdfVIP

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short-term exposure to tobacco toxins alters expression of multiple proliferation gene markers in primary human bronchial epithelial cell cultures短期接触烟草毒素改变多个增殖基因标记的表达主要人类支气管上皮细胞培养.pdf

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short-term exposure to tobacco toxins alters expression of multiple proliferation gene markers in primary human bronchial epithelial cell cultures短期接触烟草毒素改变多个增殖基因标记的表达主要人类支气管上皮细胞培养

Hindawi Publishing Corporation Journal of Oncology Volume 2011, Article ID 208563, 10 pages doi:10.1155/2011/208563 Research Article Short-Term Exposure to Tobacco Toxins Alters Expression of Multiple Proliferation Gene Markers in Primary Human Bronchial Epithelial Cell Cultures Imran S. Chaudhry,1, 2 Ashraf El-Meanawy,3, 4 Amer Khiyami,1 Joseph F. Tomashefski Jr.,1 Rhoderick N. Machekano,5 and Lawrence Kass1 1 Department of Pathology, MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH 44109, USA 2 DynaLIFE Dx, Suite 200, 10150 102 Street, Edmonton, AB, Canada T5J 5E2 3 Department of Medicine, MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH 44109, USA 4 Kidney Disease Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA 5 Center for Health Care Research and Policy, MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH 44109, USA Correspondence should be addressed to Imran S. Chaudhry, ischaudhry@ Received 18 November 2010; Accepted 3 February 2011 Academic Editor: David Z. Qian Copyright © 2011 Imran S. Chaudhry et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The biological eﬀects of only a ﬁnite number of tobacco toxins have been studied. Here, we describe exposure of cultures of human bronchial epithelial cells to low concentrations of tobacco carcinogens: nickel sulphate, benzo(b)ﬂuoranthene, N-nitrosodiethylamine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). After a 24-hour exposure, EGFR was expressed in cell membrane and cytoplasm,