significant changes in the levels of secreted cytokines in brains of experimental antiphospholipid syndrome mice重要的分泌细胞因子水平的变化实验antiphospholipid综合症小鼠的大脑.pdfVIP

Hindawi Publishing Corporation Autoimmune Diseases Volume 2012, Article ID 404815, 6 pages doi:10.1155/2012/404815 Research Article Significant Changes in the Levels of Secreted Cytokines in Brains of Experimental Antiphospholipid Syndrome Mice Assaf Menachem,1, 2, 3 Joab Chapman,1, 2, 3 and Aviva Katzav2, 3 1 Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel 2 Department of Neurology and Joseph Sagol Neuroscience Center, Sheba Medical Center, 52621 Ramat Gan, Israel 3 Sackler Faculty of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel Correspondence should be addressed to Joab Chapman, jchapman@post.tau.ac.il Received 3 October 2011; Accepted 28 November 2011 ´ Academic Editor: Jozelio Freire de Carvalho Copyright © 2012 Assaf Menachem et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Antiphospholipid syndrome (APS) is characterized by thromboses and neuropsychiatric manifestations possibly linked to brain inflammation. In order to examine the levels of proinflammatory and anti-inflammatory cytokines in experimental APS (eAPS) mice brains, we measured the levels of TNF-α, IFN-γ, and IL-10 in brain homogenates (cytosolic fractions) and in brain slices (secreted level) at 6, 15, and 24 weeks after immunization. We induced eAPS by immunization of Balb/c mice with β2 -glycoprotein I (β2 GPI), the major autoantigen in the disease and controls with adjuvant alone. We found increased levels of secreted TNF-α in eAPS mice for the entire experiment period. Cytosolic and secreted IL-10 and IFN-γ lev