small interfering rna efficiently suppresses adhesion molecule expression on pulmonary microvascular endothelium小干扰rna有效抑制肺微血管内皮细胞粘附分子的表达.pdf

SAGE-Hindawi Access to Research Journal of Nucleic Acids Volume 2011, Article ID 694789, 10 pages doi:10.4061/2011/694789 Research Article Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium Tobias Walker,1 Julian Siegel,1 Andrea Nolte,2 Silke Hartmann,3 Angela Kornberger,1 Volker Steger,1 and Hans-Peter Wendel3 1 Department of Thoracic, Cardiac and Vascular Surgery, T¨ubingen University Hospital, Hoppe-Seyler-Straße 3, 72076 T¨ubingen, Germany 2 Division of Congenital and Paediatric Cardiac Surgery, Clinical Research Laboratory, Children’s Hospital, T¨ubingen University Hospital, Hoppe-Seyler-Straße 3, 72076 T¨ubingen, Germany 3 Department of Anaesthesiology and Intensive Care, T¨ubingen University Hospital, Hoppe-Seyler-Straße 3, 72076 T¨ubingen, Germany Correspondence should be addressed to Tobias Walker, tobias.walker@med.uni-tuebingen.de Received 12 January 2011; Revised 28 February 2011; Accepted 23 March 2011 Academic Editor: Ben Berkhout Copyright © 2011 Tobias Walker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Adhesion molecules are known to influence postoperative organ function, they are hardly involved in the inflammatory response following the ischemia-reperfusion injury. We sought to investigate the potency of small interfering RNAs to suppress adhesion molecule expression in human pulmonary microvascular endothelial cells. Methods. Human lung microvascular endothelial cells were transfected with specific siRNA followed by a stimulation of the cells with an in