the effect of calcipotriol on the expression of human defensin-2 and ll-37 in cultured human keratinocytes人类defensin-2 calcipotriol对表达的影响和ll-37人类角质细胞培养.pdfVIP

Hindawi Publishing Corporation Clinical and Developmental Immunology Volume 2009, Article ID 645898, 8 pages doi:10.1155/2009/645898 Research Article The Effect of Calcipotriol on the Expression of Human β Defensin-2 and LL-37 in Cultured Human Keratinocytes Beom Joon Kim,1 Yong Kwan Rho,1 Hye In Lee,1 Mi Sook Jeong,2 Kapsok Li,1 Seong Jun Seo,1 Myeung Nam Kim,1 and Chang Kwun Hong1 1 Department of Dermatology, Chung-Ang Medical Research Center, College of Medicine, Chung-Ang University, Seoul 156-755, South Korea 2 Chung-Ang Medical Research Center, College of Medicine, Chung-Ang University, Seoul 156-755, South Korea Correspondence should be addressed to Seong Jun Seo, drseo@ Received 16 August 2009; Accepted 5 December 2009 Recommended by Yasunobu Yoshikai Background. Vitamin D has been reported to regulate innate immunity by controlling the expression of antimicrobial peptides (AMPs). Objective. We investigated the effect of calcipotriol on the expression of AMPs in human cultured keratinocytes. Methods. Keratinocytes were treated with lipopolysaccharide (LPS), TNF-α, Calcipotriol and irradiated with UVB, cultured, and harvested. To assess the expression of human beta defensin-2 and LL-37 in the control group, not exposed to any stimulants, the experimental group was treated with LPS, TNF-α, or UVB, and another group was treated again with calcipotriol; reverse transcriptase- polymerase chain reaction, Western blotting, and immunohistochemical staining were performed. Results. In the experimental group treated with LPS, UVB irradiation, and TNF-α, the expression of β -defensin and LL-37 was increased more than in the control group and then decreased in the experimental group treated with calcipotriol. Conclusions. Calcipotriol suppressed HBD-