the dynamics of cytokine d nitric oxide secretion in mice injected with tityus serrulatus scorpion venom细胞因子d一氧化氮分泌的动力学在老鼠身上注射tityus serrulatus蝎子毒液.pdfVIP

Research Communication Mediators of Inflammation, 11, 173– 180 (2002) AIMS : The effects of Tityus serrulatus venom (TSV) The dynamics of cytokine d nitric were analysed with respect to the susceptibility of four isogenic mouse, the symptoms following injec- oxide secretion in mice injected tion of venom and the inflammatory mediators in an with Tityus serrulatus scorpion experimental model of severe envenomation induced in mice. venom Methods : The susceptibility was analysed by lethal dose (LD50) determination, including the symptoms observed during envenomating and glucose levels. The detection of cytokines in serum from mice were Vera L. Petricevich1,CA and Carlos F. Pena˜ +2 analysed using enzyme-linked immunosorbent assay, and nitric oxide (NO) was analysed using nitrite determination. 1Laboratorio´ de Imunoqu´õ mica, Instituto Butantan, Results : The estimated LD50 values were in micro- Sao˜ Paulo, Brazil; 2 Instituto de Biotecnolog´õ a, UNAM grams per 100 microliters, and the susceptibility of Cuernavaca, Mexico´ mice to TSV varies with: (a) mouse strain and route of injection (A/J BALB/c C57Bl/6 = DBA); (b) mouse strain and sex (A/J female and male BALB/c female and male); and (c) body weight (all groups of A/J CACorresponding author: BALB/c groups). Tel: 005511 3726-7222; ext 223 1 Among the mouse strains studied, BALB/c mice Fax: 005511 3726-150 5 presented moderate sensibility to TSV, with changes in