the early stage of polyphosphate accumulation in saccharomyces cerevisiae comparative study by extraction and dapi staining多磷酸盐的早期阶段积累在酿酒酵母中提取和dapi染色法的比较研究.pdfVIP

Advances in Bioscience and Biotechnology, 2011, 2, 293-297 ABB doi:10.4236/abb.2011.24042 Published Online August 2011 (http://www.SciRP.org/journal/abb/). The early stage of polyphosphate accumulation in Saccharomyces cerevisiae : comparative study by extraction and DAPI staining 1 1 1 1 Lubov Ryazanova , Nadeschda Andreeva , Tatiana Kulakovskaya , Airat Valiakhmetov , Valerii Yashin2 1 1 , Vladimir Vagabov , Igor Kulaev 1Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow, Russia; 2Institute of Cell Biophysics, Russian Academy of Sciences, Moscow, Russia. Email: alla@ibpm.pushchino.ru Received 16 June 2011; revised 13 July 2011; accepted 20 July 2011. ABSTRACT tions including participation in stress response, mem- brane transport, and gene expression regulation [1-5]. Inorganic polyphosphate (PolyP), a bioactive polymer PolyP plays the key role in the processes of biominerali- with multiple functions, plays a key role in biominer- zation and phosphorus homeostasis [6,7]. The cells of alizaion and phosphorus homeostasis in yeasts. After Saccharomyces cerevisiae under certain growth condi- phosphate starvation, the cells of Saccharomyces cere- tions are able to accumulate PolyP in great amounts visiae restored their pool of PolyP during the first 30 strongly depending on the growth stage, culture condi- min of incubation in the media contai