the effect of dosage, gestational age and splenectomy on anti-igm interception of prenatal b-cell development in sheep剂量的影响,胎龄和脾切除术anti-igm拦截产前b细胞发展的羊.pdfVIP

Clinical Developmental Immunology, March 2003, Vol. 10 (1), pp. 19–26 The Effect of Dosage, Gestational Age and Splenectomy on Anti-IgM Interception of Prenatal B-cell Development in Sheep a b, c d b P. MCCULLAGH , C.MCL. PRESS *, S.J. MCCLURE , H.J. LARSEN and T. LANDSVERK aJohn Curtin School of Medical Research, Australian National University, Canberra, ACT 2600, Australia; bDepartment of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science, P.O. Box 8146, Dep. 0033, Oslo, Norway; cCSIRO Livestock Industries McMaster Laboratory, Armidale, NSW 2350, Australia; dDepartment of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, Oslo, Norway The administration of a single bolus of anti-IgM antibody to foetal lambs early in pregnancy produces prolonged B-cell depletion. The present study investigated this depletion by examining the effect, on B-cell development in the ileal Peyer’s patches, of varying the timing and dosage of antibody administration and by supplementing anti-IgM with surgical splenectomy. The capacity of a 1 mg bolus of anti-IgM to deplete Peyer’s patches of B cells was lost if its administration was deferred until two thirds of the way through pregnancy, but persisted beyond this time if weight-adjusted doses were used. Splenectomy of the foetus performed at an earlier age failed to extend the age at which a 1 mg dose of antibody remained effective. As the concentration of murine immunoglobulin in foetal serum was greatly reduced afte