三疣梭子蟹(Portunus+trituberculatus)在氨氮胁迫下解毒代谢机制的研究.pdfVIP

Study on detoxification metabolic mechanism of swimming crab Portunus trituberculatus exposed to ammonia Abstract Ammonia is a major threat factor in aquaculture ambient water in pond. In this study, some key detoxification-related genes were cloned and characterized from the gills and hepatopancreas of swimming crab Portunus trituberculatus. The effects of ammonia on ammonia transportation indicators, key detoxification-related gene expression and ammonia metabolic parameters were determined to detect the ammonia detoxification metabolism mechanism in the swimming crab Portunus trituberculatus. The study mainly includes three bodies as follows: (1) Cloning characterization and expression analysis of key detoxification-related genes in swimming crab P. trituberculatus, including Rh protein and glutamate dehydrogenase (GDH); (2) Effects of ammonia on ammonia transportation pathway in swimming crab P. trituberculatus; (3) Effects of ammonia on ammonia metabolic pathway in swimming crab P. trituberculatus. The results are as follows: 1 Cloning, characterization and expression analysis of detoxification-related genes in swimming crab P. trituberculatus The partial cDNA sequences of Rh protein and GDH were cloned from the gills and hepatopancreas in swimming crab P. trituberculatus using degenerate primer and RT-PCR methods. The partial cDNA of Rh protein was of 612 bp encoding 204 amino acids. The Rh protein amino acids showed 86%-91% high similarity to other crustacean Rh protein. In addition, the partial cDNA of GDH was of 414