原创力文档a conserved ga element in tata-less rna polymerase ii promoters守恒的ga元素tata-less rna聚合酶ii推动者.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
a conserved ga element in tata-less rna polymerase ii promoters守恒的ga元素tata-less rna聚合酶ii推动者
A Conserved GA Element in TATA-Less RNA Polymerase II
Promoters
. . . ¤
Martin Seizl , Holger Hartmann , Friederike Hoeg , Fabian Kurth , Dietmar E. Martin, Johannes
¨
Soding*, Patrick Cramer*
¨ ¨
Department of Biochemistry and Gene Center, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universitat (LMU) Munchen, Munich, Germany
Abstract
Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In
the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a
TATA box occurs in only 15% of promoters in the yeast Saccharomyces cerevisiae, posing the question how most yeast
promoters nucleate PIC assembly. Here we show that one third of all yeast promoters contain a novel conserved DNA
element, the GA element (GAE), that generally does not co-occur with the TATA box. The distance of the GAE to the
transcription start site (TSS) resembles the distance of the TATA box to the TSS. The TATA-less TMT1 core promoter contains
a GAE, recruits TBP, and supports formation of a TBP-TFIIB-DNA-complex. Mutation of the promoter region surrounding the
GAE abolishes transcription in vivo and in vitro. A 32-nucleotide promoter region containing the GAE can functionally
substitute for the TATA box in a TATA-containing promoter. This identifies the GAE as a conserved promoter element in
TATA-less promoters.
Citation: Seizl M, Hartmann H, Hoeg F, Kurth F, Martin DE, et al. (2011) A Conserved GA Element in TATA-Less RNA Polymer
您可能关注的文档
- (a)symmetric stem cell replication and cancer(一)对称复制干细胞和癌症.pdf
- 1,25-dihydroxyvitamin d3 promotes a sustained lps-induced nf-κb-dependent expression of cd55 in human monocytic thp-1 cells1,25-dihydroxyvitamin d3促进持续lps-induced nf-κb-dependent人类单核细胞的表达cd55 thp-1细胞.pdf
- 2nd international external quality control assessment for the molecular diagnosis of dengue infections第二国际的外部质量控制评估的分子诊断登革热感染.pdf
- 1,25-dihydroxyvitamin d3 inhibits the differentiation and migration of th17 cells to protect against experimental autoimmune encephalomyelitis1,25-dihydroxyvitamin d3抑制th17细胞的分化和迁移,防止实验性自身免疫性脑脊髓炎.pdf
- 2,4-diaminopyrimidines as potent inhibitors of trypanosoma brucei and identification of molecular targets by a chemical proteomics approach2,4-diaminopyrimidines强有力的抑制剂的锥虫属brucei化学蛋白质组学和分子靶点的识别方法.pdf
- 1+1？=？3 a fusion of 2 enzymes in the methionine salvage pathway of tetrahymena thermophila creates a trifunctional enzyme that catalyzes 3 steps in the pathway1 + 1 = 3融合2蛋氨酸救助途径的酶四膜虫thermophila创建一个三功能性的酶,这种酶催化途径3步骤.pdf
- 3, 3′5 triiodo l thyronine induces apoptosis in human breast cancer mcf-7cells, repressing smp30 expression through negative thyroid response elements3、3u20325 l三碘甲状腺原氨酸人类乳腺癌mcf-7cells发生凋亡,抑制smp30表达式通过负甲状腺响应元素.pdf
- 3′utr-mediated gene silencing of the mixed lineage leukemia (mll) gene3u2032utr-mediated基因沉默的混合血统白血病mll基因.pdf
- 3d active stabilization system with sub-micrometer resolution3 d与sub-micrometer决议主动稳定系统.pdf
- (s)-α-chlorohydrin inhibits protein tyrosine phosphorylation through blocking cyclic amp - protein kinase a pathway in spermatozoa(s)-α-chlorohydrin抑制蛋白质酪氨酸磷酸化通过阻断环腺苷酸蛋白激酶精子的途径.pdf
- a conserved gene structure and expression regulation of mir-433 and mir-127 in mammals保守基因结构和表达调控mir - 433和mir - 127的哺乳动物.pdf
- a condensation-ordering mechanism in nanoparticle-catalyzed peptide aggregationcondensation-ordering机制在nanoparticle-catalyzed肽聚合.pdf
- a conserved function of c. elegans casy-1 calsyntenin in associative learning秀丽隐杆线虫的守恒函数casy-1 calsyntenin联想学习.pdf
- a computer simulation of progesterone and cox2 inhibitor treatment for preterm labor计算机模拟的孕激素和cox2抑制剂治疗早产.pdf
- a conserved tissue-specific homeodomain-less isoform of meis1 is downregulated in colorectal cancer保守组织homeodomain-less同种型meis1大肠癌中表达下调.pdf
- a conserved non-reproductive gnrh system in chordates激性腺素释放素系统守恒的非生殖脊索动物.pdf
- a constructed alkaline consortium and its dynamics in treating alkaline black liquor with very high pollution load碱性财团构造及其动力学治疗碱性黑液与很高的污染负荷.pdf
- a conserved developmental patterning network produces quantitatively different output in multiple species of drosophila守恒的发育模式网络定量产生不同的输出在多个种类的果蝇.pdf
- a contracted dna repeat in lhx3 intron 5 is associated with aberrant splicing and pituitary dwarfism in german shepherd dogs简约的dna重复lhx3内含子5与德国牧羊犬异常剪接和垂体性侏儒症.pdf
- a cooperative interaction between nontranslated rna sequences and ns5a protein promotes in vivo fitness of a chimeric hepatitis cgb virus b合作互动nontranslated rna序列和ns5a蛋白促进体内嵌合cgb型肝炎病毒b的健身.pdf
文档评论（0）