甲醇利用型吡咯喹啉醌产生菌的筛选及鉴定-生物技术通报.PDF

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 年第 期 2013 1 甲醇利用型吡咯喹啉醌产生菌的筛选及鉴定 徐文  许然  张利平 （河北大学生命科学学院 河北省微生物多样性研究与应用重点实验室，保定 071002） 摘 要 ： 从虎杖内生细菌和黏细菌中筛选吡咯喹啉醌 （PQQ ）产生菌。采用3 种以甲醇为唯一碳源的培养基对160 株供试 菌株进行摇瓶培养发酵，发酵产物采用光谱学分析法及HPLC 法筛选。结果显示，通过初筛和复筛共得到甲醇利用型菌134 株， PQQ 产生菌4 株，其中菌株083114 的PQQ 产量为64.34 mg/L。菌株083114 的16S rRNA 基因序列分析结果显示，其序列与酸快生 芽孢杆菌 （ ）的系统发育关系最近。虎杖内生细菌及黏细菌中存在PQQ 产生菌。 Bacillus acidiceler 关键词 ： 甲醇利用型菌 吡咯喹啉醌 内生细菌 黏细菌 光谱法 Isolation and Identification of PQQ Producing Strains Using - Methanol utilizing Bacteria Xu Wen Xu Ran Zhang Liping （Key Laboratory of Microbial Diversity Research and App lication of Hebei Province ，College of Lif e Sciences ，Hebei University ， Baoding 07 1002 ） Abstract:  The aim was to isolate Pyrroloquinoline quinine producing strains from endophytic bacteria of Polygonum cuspidatum and strains of myxobacteria. Three kinds of media with methanol as single carbon source were used for microbial fermentation of the strains. Spectroscopy and HPLC analysis of the fermentation product were performed. Through preliminary screening and complex screening we got 134 strains of methanol-utilizing and 4 strains of Pyrroloquinoline quinine producing. The Pyrroloquinoline quinine producing strain 083114 was determined to be 64.34 mg/L. The 16S rRNA sequence analysis showed that the phylogenetic relationship between strain 083114 and Bacillus acidiceler is t