幽门螺杆菌粪便抗原检测临床应用.docVIP

幽门螺杆菌粪便抗原检测临床应用【摘要】 目的：评价幽门螺杆菌粪便抗原(HpSA)检测诊断幽门螺杆菌感染的特异性和敏感性。方法：采用酶联免疫分析法检测150例反复腹痛或伴有恶心、呕吐等上消化道症状患儿的幽门螺杆菌粪便抗原，同时以13C尿素呼气试验 (13C-UBT)检查作对照，对HpSA酶联免疫法与13C尿素呼气试验(13C-UBT)检查的阳性结果进行比较。结果：150例患儿中，HpSA酶联免疫法阳性率30%，对照法阳性率为29.33%，两法阳性检测率比较差异无统计学意义，HpSA酶联免疫法与对照法比较其敏感度和特异度分别为84.09%和92.45%。结论：HpSA酶联免疫法是一种操作简便、安全、可靠的诊断幽门螺杆菌感染的方法，尤其适用于儿童幽门螺杆菌感染的普查。 【关键词】 幽门螺杆菌； 粪便抗原； 酶联免疫法 Clinical Application of Helicobacter Pylori Feces Antigen Detection/ZHOU Ning，LI Dong-mei，YE Jia-wei.//Medical Innovation of China，2012，9(14):084-085 【Abstract】 Objective:To evaluation specificity and sensitivity of Helicobacter pylori stool antigen (HpSA) detection in diagnosis of Helicobacter pylori infection.Methods:Enzyme-linked immunosorbent assay of 150 cases of recurrent abdominal pain or nausea， vomiting and other gastrointestinal symptoms in children on the Helicobacter pylori stool antigen， while 13C urea breath test (13C-UBT) for the control check， the HpSA enzyme-linked immunoassay and 13C urea breath test (13C-UBT)-positive results were compared to evaluate the HpSA diagnostic sensitivity and specificity of Helicobacter pylori infection. Results:150 cases in children，HpSA ELISA-positive rate of 30%， the positive rate was 29.33% control method， the positive detection rate of these two methods was no significant statistical difference， HpSA ELISA method were compared with the sensitivity and specificity degrees were 84.09% and 92.45%.Conclusion:HPSA ELISA is a simple， safe and reliable method of diagnosis of Helicobacter pylori infection， especially for Helicobacter pylori infection in the census. 【Key words】 Helicobacter pylori； Stool antigen； Enzyme-linked immunosorbent assay First-authors address:Maternal and Child Hospital of Haizhu District in Guang Zhou，Guangzhou 510240，China doi：10.3969/j.issn.1674-4985.2012.14.054 幽门螺杆菌(Hp)属于革兰氏阴性细菌，可定植于胃黏膜并可引起胃十二指肠疾病。幽门螺杆菌(Helicobacterpylori，Hp)感染是慢性胃炎、消化性溃疡的重要病因，且与胃癌、胃黏膜相关性淋巴样组织(MALT)淋巴瘤密切相关[1-2]。目前检测Hp感染的方法有侵入性检查和非侵入性检查两种，其根据是是否通过胃镜检