舒胸片中人参皂苷Rg1-人参皂苷Rb1-三七皂苷R1含量测定.docVIP

舒胸片中人参皂苷Rg1\人参皂苷Rb1\三七皂苷R1含量测定[摘要] 目的:建立高效液相法同时测定舒胸片中人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1的含量测定方法。方法:采用Shim-pack-C18(4.6 mm×250 mm,5 μm)色谱柱;流动相:乙腈-水进行梯度洗脱;检测波长:203 nm;流速:1.0 ml/min。结果:人参皂苷Rg1在0.82～8.20 μg范围内与峰面积呈良好的线性关系(r=0.999 8);人参皂苷Rb1在0.88～8.80 μg范围内与峰面积呈良好的线性关系(r=0.999 6);三七皂苷R1在0.32～3.20 μg范围内与峰面积呈良好的线性关系(r=0.999 8),总皂苷的平均回收率为99.76%,RSD=1.26%(n=6)。结论:本方法简便、准确、重现性好,可用于舒胸片中总皂苷的质量控制。 [关键词] 高效液相法;舒胸片;人参皂苷Rg1;人参皂苷Rb1;三七皂苷R1;含量测定 [中图分类号] R927.2[文献标识码] B[文章编号] 1673-7210(2010)04(a)-084-02 Content determination of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in Shuxiong Tablets by RP-HPLC LIU Wei1, WU Yizhong 2 (1.Guangzhou Chenliji Pharmaceutical Co., Guangdong Province, Guangzhou 510290, China; 2.Guangzhou Pharmaceutical Holdings Co. Ltd., Guangdong Province, Guangzhou 510130, China) [Abstract] Objective: To establish a method for the content determination of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in Shuxiong Tablets by RP-HPLC. Methods: The Shim-pack-C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phases consisted of acetonitrile and water (gradient elution), the flow rate was 1.0 ml/min and the detection wavelength was at 203 nm. Results: The linear ranges of ginsenoside Rg1 was at 0.82-8.20 μg; the linear ranges of ginsenoside Rb1 was at 0.88-8.80 μg; the linear ranges of notoginsenoside R1 was at 0.32-3.20 μg. The average recovery was 99.76% with a RSD of 1.26%. Conclusion: The method is fast, reliable, accurate and can be used in the quality control of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside in Shuxiong Tablets. [Key words] HPLC; Shuxiong Tablets; Ginsenoside Rg1; Ginsenoside Rb1; Notoginsenoside R1; Content determination 舒胸片由三七、红花、川芎三味药材组成,具有活血化瘀、通络止痛的功效,临床用于瘀血阻滞所致的胸痹、胸闷、心前区刺痛及冠心病心绞痛等症状的治疗。舒胸片质量标准收载于《中国药典》2005年版一部,原标准对处方中川芎、三七进行薄层鉴别,并采用薄层扫描法测定三七中人参皂苷Rg1的含量。笔者为提高舒胸片的质量标准,参考文献[1~8]用HPLC法测定三七中总皂苷的含量。 1 仪器与试药 1.1仪器 Agilent 1100液相色谱仪(UV检测器,Agilent 10.02色谱工作站)。 1.2 试药 人参皂苷Rg1(批号:110703-200425,中国药品