铁皮石斛内生真菌分布.pdfVIP

微生物学通报 JAN 20, 2010, 37(1): 3742 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 铁皮石斛内生真菌分布 胡克兴 侯晓强 郭顺星* ( 中国医学科学院 北京协和医学院 药用植物研究所 北京 100193) 摘 要: 本研究从铁皮石斛(Dendrobium officinale)根、茎和叶中分离得到内生真菌 67 株, 经形态 学和分子生物学鉴定分别归属于16 个属, Fusarium 和Alternaria 为铁皮石斛内生真菌的优势种群。 研究发现, 铁皮石斛根、茎、叶中内生真菌分布存在较大差别, 具有一定的组织差异性, 不同石斛 植物中内生真菌的存在情况差别较大, 具有一定的宿主差异性。本研究丰富了真菌资源, 为石斛 属药用植物内生真菌资源的开发利用提供了基础理论依据。 关键词: 铁皮石斛, 内生真菌, 鉴定, 分布 Distribution of Endophytic Fungi in Dendrobium officinale * HU Ke-Xing HOU Xiao-Qiang GUO Shun-Xing (Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100193, China) Abstract: A total of 67 strains of endophytic fungi were isolated from roots, branches and leaves in Den- drobium officinale. These strains were identified into 16 genera based on morphological characters and ri- bosomal DNA(rDNA) sequence analysis, thereinto Fusarium and Alternaria are dominant populations in D . officinale. From analysis results we found some endophytic fungi show a certain degree of host and tissue preference. The research provided theoretical basis for developing and utilizing the resources of endophytic fungi in Dendrobium . Keywords: Dendrobium officinale, Endophytic fungi, Identification, Distribution 铁 皮 石 斛 (Dendrobium officinale) 为 石 斛 属 起步较晚, 此前国内外只有范黎[6] 、朱国胜[7] 、张廷 (De