bcg vaccination a role for vitamin dbcg接种维生素d的作用.pdfVIP

BCG Vaccination: A Role for Vitamin D? 1 2 1 1 1 2 Maeve K. Lalor *, Sian Floyd , Patricia Gorak-Stolinska , Rosemary E. Weir , Rose Blitz , Keith Branson , Paul E. Fine2, Hazel M. Dockrell1 1 Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene Tropical Medicine, London, United Kingdom, 2 Department of Infectious Disease Epidemiology, Faculty of Epidemiology and Public Health, London School of Hygiene Tropical Medicine, London, United Kingdom Abstract Background: BCG vaccination is administered in infancy in most countries with the aim of providing protection against tuberculosis. There is increasing interest in the role of vitamin D in immunity to tuberculosis. This study objective was to determine if there was an association between circulating 25(OH)D concentrations and BCG vaccination status and cytokine responses following BCG vaccination in infants. Methods: Blood samples were collected from UK infants who were vaccinated with BCG at 3 (n = 47) and 12 (n = 37) months post BCG vaccination. These two time-points are denoted as time-point 1 and time-point 2. Two blood samples were also collected from age-matched unvaccinated infants (n = 32 and 28 respectively), as a control group. Plasma vitamin D concentrations (25(OH)D) were measured by radio-immunoassay. The cytokine IFNc was measured in supernatants from diluted whole blood stimulated with M.tuberculosis (M.tb) PPD for 6 days. Results: 58% of infants had some level of hypovitaminosis (25(OH)D ,30ng/ml) at time-point 1, and this increased to 97% 9 months later. BCG vaccinated infants were almost 6 times (CI: 1.8–18.6) more likely to have suf