capillary electrophoresis separation of protein composition of γ-irradiated food pathogens listeria monocytogenes and staphylococcus aureus毛细管电泳分离的蛋白质组成γ-irradiated食物病原体单核细胞增多性李斯特氏菌和金黄色葡萄球菌.pdfVIP

Capillary Electrophoresis Separation of Protein Composition of c-Irradiated Food Pathogens Listeria monocytogenes and Staphylococcus aureus 1 1 2 1 Karine Trudeau , Khanh Dang Vu , Franc¸ois Shareck , Monique Lacroix * ´ 1 INRS-Institut Armand-Frappier, Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre, Laval, Quebec, Canada, 2 INRS-Institut Armand-Frappier, ´ Laval, Quebec, Canada Abstract Background: A capillary electrophoresis method using UV detection was developed to analyse protein composition of the lysates of two foodborne pathogens, Listeria monocytogenes and Staphylococcus aureus which were previously treated at different irradiation doses. Methodology and Principal Findings: Bacterial samples were c-irradiated at different doses to produce damage cells, to kill cells and to provoke viable but non culturable cells (VBNC) in order to evaluate the respective expression of stress proteins. In Listeria monocytogenes, two proteins (MW of 70.2 and 85.4 kDa) were significantly changed (P#0.05) at different doses of irradiation. In Staphyloccocus aureus, one protein (50 S ribosomal protein) with the MW of 16.3 kDa was significantly decreased at a low dose of irradiation treatment and the other protein (transcriptional regulator CtsR) with the MW of 17.7 kDa was increased significantly (P#0.05) at all doses of irradiation treatment compared to control. Conclusion: Expression of two proteins from the acyltransferase family in Listeria monocytogenes was statistically changed during irradiation treatment (P#0.05). In Staphylococcus aureus, expression of the 50 S ribosomal protein decr