capsaicin displays anti-proliferative activity against human small cell lung cancer in cell culture and nude mice models via the e2f pathway辣椒素显示anti-proliferative活动对人类小细胞肺癌细胞培养和裸小鼠模型通过e2f通路.pdfVIP

Capsaicin Displays Anti-Proliferative Activity against Human Small Cell Lung Cancer in Cell Culture and Nude Mice Models via the E2F Pathway 1. 2. 2 3 3 4 Kathleen C. Brown , Ted R. Witte , W. Elaine Hardman , Haitao Luo , Yi C. Chen , A. Betts Carpenter , 1 1 Jamie K. Lau , Piyali Dasgupta * 1 Department of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, United States of America, 2 Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, United States of America, 3 Department of Biology, Alderson-Broaddus College, Phillipi, West Virginia, United States of America, 4 Department of Anatomy and Pathology, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, United States of America Abstract Background: Small cell lung cancer (SCLC) is characterized by rapid progression and low survival rates. Therefore, novel therapeutic agents are urgently needed for this disease. Capsaicin, the active ingredient of chilli peppers, displays anti- proliferative activity in prostate and epidermoid cancer in vitro. However, the anti-proliferative activity of capsaicin has not been studied in human SCLCs. The present manuscript fills this void of knowledge and explores the anti-proliferative effect of capsaicin in SCLC in vitro and in vivo. Methodology/Principal Findings: BrdU assays and PCNA ELISAs showed that capsaicin displays robust anti-proliferative activity in four human SCLC cell lines. Furthermore, capsaicin potently suppressed the growth of H69 human SCLC tumors in vivo as