cellular levels of hiv unspliced rna from patients on combination antiretroviral therapy with undetectable plasma viremia predict the therapy outcome细胞水平的hiv unspliced rna与联合抗逆转录病毒疗法患者察觉血浆病毒血症预测治疗的结果.pdfVIP

Cellular Levels of HIV Unspliced RNA from Patients on Combination Antiretroviral Therapy with Undetectable Plasma Viremia Predict the Therapy Outcome 1 2 1 3 1 Alexander O. Pasternak , Suzanne Jurriaans , Margreet Bakker , Jan M. Prins , Ben Berkhout , Vladimir V. Lukashov1* 1 Department of Medical Microbiology, Laboratory of Experimental Virology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands, 2 Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands, 3 Department of Internal Medicine, Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands Abstract Background: Combination antiretroviral therapy (cART), the standard of care for HIV-1 infection, is considered to be successful when plasma viremia remains below the detection limit of commercial assays. Yet, cART fails in a substantial proportion of patients after the apparent success. No laboratory markers are known that are predictive of cART outcome in initial responders during the period of undetectable plasma viremia. Methodology/Principal Findings: Here, we report the results of a retrospective longitudinal study of twenty-six HIV- infected individuals who initially responded to cART by having plasma viremia suppressed to ,50 copies/ml. Eleven of these patients remained virologically suppressed, whereas fifteen experienced subsequent cART failure. Using sensitive methods based on seminested real-time PCR, we measured the levels of HIV-1 proviral (pr) DNA, unspl