characterization and identification of subpopulations of mononuclear preosteoclasts induced by tnf-α in combination with tgf-β in rats引起的亚种群的特征和识别单核preosteoclasts tnf-α结合tgf-β老鼠.pdfVIP

Characterization and Identification of Subpopulations of Mononuclear Preosteoclasts Induced by TNF-a in Combination with TGF-b in Rats 1,2 3 1 1 3 1,4 Rei Matsubara , Toshio Kukita , Yuka Ichigi , Ippei Takigawa , Peng-Fei Qu , Noboru Funakubo , 1 2 1 Hiroshi Miyamoto , Kazuaki Nonaka , Akiko Kukita * 1 Department of Microbiology, Faculty of Medicine, Saga University, Nabeshima, Saga, Japan, 2 Pediatric Dentistry, Faculty of Dental Science, Kyushu University, Maidashi, Fukuoka, Japan, 3 Molecular Cell Biology and Oral Anatomy, Kyushu University, Maidashi, Fukuoka, Japan, 4 Oral and Maxillofacial Oncology, Kyushu University, Maidashi, Fukuoka, Japan Abstract Osteoclasts are unique multinucleated cells formed by fusion of preosteoclasts derived from cells of the monocyte/ macrophage lineage, which are induced by RANKL. However, characteristics and subpopulations of osteoclast precursor cells are poorly understood. We show here that a combination of TNF-a, TGF-b, and M-CSF efficiently generates mononuclear preosteoclasts but not multinucleated osteoclasts (MNCs) in rat bone marrow cultures depleted of stromal cells. Using a rat osteoclast-specific mAb, Kat1, we found that TNF-a and TGF-b specifically increased Kat1+c-fms+ and + 2 2 + 2 + + + Kat1 c-fms cells but not Kat1 c-fms cells. Kat1 c-fms cells appeared in early stages of culture, but Kat1 c-fms and Kat1+c-fms