cardiomyocyte formation by skeletal muscle-derived multi-myogenic stem cells after transplantation into infarcted myocardium心肌细胞形成的骨胳肌源性multi-myogenic干细胞移植后心肌梗塞.pdfVIP

Cardiomyocyte Formation by Skeletal Muscle-Derived Multi-Myogenic Stem Cells after Transplantation into Infarcted Myocardium Tetsuro Tamaki1,2*, Akira Akatsuka1,3, Yoshinori Okada1,3, Yoshiyasu Uchiyama1,4, Kayoko Tono1,2, Mika 2 1,5 2 6 6 2,6 Wada , Akio Hoshi , Hideki Iwaguro , Hiroto Iwasaki , Akira Oyamada , Takayuki Asahara 1 Muscle Physiology Cell Biology Unit, Tokai University School of Medicine, Isehara, Kanagawa, Japan, 2 Department of Regenerative Medicine, Division of Basic Clinical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan, 3 Teaching Research Support Center, Tokai University School of Medicine, Isehara, Kanagawa, Japan, 4 Department of Orthopedics, Division of Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan, 5 Department of Urology, Division of Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan, 6 Stem Cell Translational Research, Kobe Institute of Biomedical Research and Innovation/RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan Abstract Background: Cellular cardiomyoplasty for myocardial infarction has been developed using various cell types. However, complete differentiation and/or trans-differentiation into cardiomyocytes have never occurred in these transplant studies, whereas functional contributions were reported. + 2 Methods and Results: Skeletal muscle interstitium-derived CD34 /CD45 (Sk-34) cells were purified from green fluorescent protein transgenic mice by flowcytometory. Cardiac differentiation of Sk-34 cells was examined by in vitro clonal culture and co-culture with embryonic cardiomyocytes, and in vivo transplantation into a