collagenolytic activities of the major secreted cathepsin l peptidases involved in the virulence of the helminth pathogen, fasciola hepatica溶胶原的活动的主要分泌组织蛋白酶l肽酶参与寄生虫病原体的毒性,肝片吸虫.pdfVIP

Collagenolytic Activities of the Major Secreted Cathepsin L Peptidases Involved in the Virulence of the Helminth Pathogen, Fasciola hepatica 1 2 1 1 1 Mark W. Robinson *, Ileana Corvo , Peter M. Jones , Anthony M. George , Matthew P. Padula , Joyce 1 2¤a 2¤b 2 2 3 To , Martin Cancela , Gabriel Rinaldi , Jose F. Tort , Leda Roche , John P. Dalton ´ 1 Infection, Immunity and Innovation (i3) Institute, University of Technology Sydney (UTS), Sydney, New South Wales, Australia, 2 Departmento de Genetica, Facultad de ´ Medicina, Universidad de la Republica (UDELAR), Montevideo, Uruguay, 3 Institute of Parasitology, McGill University, St. Anne de Bellevue, Quebec, Canada Abstract Background: The temporal expression and secretion of distinct members of a family of virulence-associated cathepsin L cysteine peptidases (FhCL) correlates with the entry and migration of the helminth pathogen Fasciola hepatica in the host. Thus, infective larvae traversing the gut wall secrete cathepsin L3 (FhCL3), liver migrating juvenile parasites secrete both FhCL1 and FhCL2 while the mature bile duct parasites, which are obligate blood feeders, secrete predominantly FhCL1 but also FhCL2. Methodology/Principal Findings: Here we show that FhCL1, FhCL2 and FhCL3 exhibit differences in their kinetic parameters towards a range of peptide substr