collective dynamics of active cytoskeletal networks集体动力学的活跃细胞骨架网络.pdfVIP

Collective Dynamics of Active Cytoskeletal Networks ¨ Simone Kohler, Volker Schaller, Andreas R. Bausch* ¨ ¨ E27 Cellular Biophysics, Technische Universitat Munchen, Garching, Germany Abstract Self organization mechanisms are essential for the cytoskeleton to adapt to the requirements of living cells. They rely on the intricate interplay of cytoskeletal filaments, crosslinking proteins and molecular motors. Here we present an in vitro minimal model system consisting of actin filaments, fascin and myosin-II filaments exhibiting pulsatile collective dynamics and superdiffusive transport properties. Both phenomena rely on the complex competition of crosslinking molecules and motor filaments in the network. They are only observed if the relative strength of the binding of myosin-II filaments to the actin network allows exerting high enough forces to unbind actin/fascin crosslinks. This is shown by varying the binding strength of the acto-myosin bond and by combining the experiments with phenomenological simulations based on simple interaction rules. ¨ Citation: Kohler S, Schaller V, Bausch AR (2011) Collective Dynamics of Active Cytoskeletal Networks. PLoS ONE 6(8): e23798. doi:10.1371/journal.pone.0023798 Editor: Daniel J. Muller, Swiss Federal Institute of Technology Zurich, Switzerland Received May 11, 2011; Accepted July 25, 2011; Published August 26, 2011 ¨ Copyright: 2011 Kohler et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The authors thank the DFG in the framework