comparative functional analysis of the caenorhabditis elegans and drosophila melanogaster proteomes对比功能分析的秀丽隐杆线虫和果蝇蛋白质组.pdfVIP

PLoS BIOLOGY Comparative Functional Analysis of the Caenorhabditis elegans and Drosophila melanogaster Proteomes Sabine P. Schrimpf1,2*, Manuel Weiss1,2,3, Lukas Reiter1,2,3,4, Christian H. Ahrens2,5, Marko Jovanovic1,2,3, ¨ 4 2 2,4 6 5 4,7 Johan Malmstrom , Erich Brunner , Sonali Mohanty , Martin J. Lercher , Peter E. Hunziker , Ruedi Aebersold , Christian von Mering1,2,8*, Michael O. Hengartner1,2* 1 Institute of Molecular Biology, University of Zurich, Zurich, Switzerland, 2 Center for Model Organism Proteomes, University of Zurich, Zurich, Switzerland, 3 PhD Program in Molecular Life Sciences, University of Zurich, Zurich, Switzerland, 4 Institute of Molecular Systems Biology, Swiss Federal Institute of Technology Zurich, Zurich, Switzerland, ¨ 5 Functional Genomics Center, University of Zurich and Swiss Federal Institute of Technology Zurich, Zurich, Switzerland, 6 Institute of Informatics, University of Dusseldorf, ¨ Dusseldorf, Germany, 7 Institute for Systems Biology, Seattle, Washington, United States of America, 8 Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland The nematode Caenorhabditis elegans is a popular model system in genetics, not least because a majority of human disease genes are conserved in C. elegans. To generate a comprehensive inventory of its expressed proteome, we performed extensive shotgun proteomics and identified more than half of all predicted C. elegans proteins. This allowed us to confirm and