comparative genomics of bordetella pertussis reveals progressive gene loss in finnish strains百日咳博德特氏菌的比较基因组学揭示了进步在芬兰菌株基因损失.pdfVIP

Comparative Genomics of Bordetella pertussis Reveals Progressive Gene Loss in Finnish Strains 1. 1,2. 3 3 4 4 3 5 1 Eriikka Heikkinen , Teemu Kallonen , Lilli Saarinen , Rolf Sara , Audrey J. King , Frits R. Mooi , Juhani T. Soini , Jussi Mertsola , Qiushui He * 1 Pertussis Reference Laboratory, National Public Health Institute, Turku, Finland, 2 Turku Graduate School of Biomedical Sciences, University of Turku, ˚ Turku, Finland, 3 Finnish DNA Microarray Centre, Turku Centre for Biotechnology, University of Turku, Abo Akademi University, Turku, Finland, 4 Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands, 5 Department of Pediatrics, Turku University Hospital, Turku, Finland Background. Bordetella pertussis is a Gram-negative bacterium that infects the human respiratory tract and causes pertussis or whooping cough. The disease has resurged in many countries including Finland where the whole-cell pertussis vaccine has been used for more than 50 years. Antigenic divergence has been observed between vaccine strains and clinical isolates in Finland. To better understand genome evolution in B. pertussis circulating in the immunized population, we developed an oligonucleotide-based microarray for comparative genomic analysis of Finnish strains isolated during the period of 50 years. Methodology/Principal Findings. The microarray consisted of 3,582 oligonucleotides (70-mer) and covered 94% of 3,816 ORFs of Tohama I, the strain of which the genome has been sequenced [1]. Twenty isolates from 1953 to 2004 were studied together with two Finn