comparative genotyping of campylobacter jejuni strains from patients with guillain-barré syndrome in bangladesh比较基因分型的空肠弯曲杆菌菌株在孟加拉国格林-巴利综合征患者.pdfVIP

Comparative Genotyping of Campylobacter jejuni Strains ´ from Patients with Guillain-Barre Syndrome in Bangladesh 1,2 2 3,4 5 3 Zhahirul Islam *, Alex van Belkum , Jaap A. Wagenaar , Alison J. Cody , Albert G. de Boer , Helen 6 2 1 1,2 Tabor , Bart C. Jacobs , Kaisar A. Talukder , Hubert P. Endtz 1 International Centre for Diarrheal Diseases Research, Dhaka, Bangladesh, 2 Erasmus MC, Rotterdam, The Netherlands, 3 Central Veterinary Institute of Wageningen UR, Lelystad, The Netherlands, 4 Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands, 5 Department of Zoology, University of Oxford, Oxford, United Kingdom, 6 National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, LCDC, Winnipeg, Canada Abstract Background: Campylobacter jejuni is a common cause of acute gastroenteritis and is associated with post-infectious ´ neuropathies such as the Guillain-Barre syndrome (GBS) and the Miller Fisher syndrome (MFS). We here present comparative genotyping of 49 C. jejuni strains from Bangladesh that were recovered from patients with enteritis or GBS. All strains were serotyped and analyzed by lipo-oligosaccharide (LOS) genotyping, amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Methodology/Principal Findings: C. jejuni HS:23 was a predominant serotype among GBS patients (50%), and no specific serotype was significantly associated with GBS