circulating endothelial cells and microparticles as prognostic markers in advanced non-small cell lung cancer循环内皮细胞和微粒子在先进的非小细胞肺癌预后标记.pdfVIP

Circulating Endothelial Cells and Microparticles as Prognostic Markers in Advanced Non-Small Cell Lung Cancer 1 ´ 2 2 2 2 Tania Fleitas *, Vicenta Martınez-Sales , Virtudes Vila , Edelmiro Reganon , David Mesado , ´ 1 ´ ´ 1 ´ 1 ´ 1 Maria Martın , Jose Gomez-Codina , Joaquın Montalar , Gaspar Reynes ´ ´ 1 Department of Medical Oncology, Hospital Universitario y Politecnico La Fe Valencia, Valencia, Spain, 2 Research Center,Hospital Universitario y Politecnico La Fe Valencia, Valencia, Spain Abstract Background: Circulating endothelial cells and microparticles have prognostic value in cancer, and might be predictors of response to chemotherapy and antiangiogenic treatments. We have investigated the prognostic value of circulating endothelial cells and microparticles in patients treated for advanced non-small cell lung cancer. Methodology/Principal Findings: Peripheral blood samples were obtained from 60 patients before first line, platinum- based chemotherapy +/ 2 bevacizumab, and after the third cycle of treatment. Blood samples from 60 healthy volunteers were also obtained as controls. Circulating endothelial cells were measured by an immunomagnetic technique and immunofluorescence microscopy. Phosphatidylserine-positive microparticles were evaluated by flow cytometry. Micropar- ticle-mediated proc