changes to the natural killer cell repertoire after therapeutic hepatitis b dna vaccination改变自然杀伤细胞治疗乙肝dna疫苗接种后曲目.pdfVIP

Changes to the Natural Killer Cell Repertoire after Therapeutic Hepatitis B DNA Vaccination 1 2,3 ´ ` 4 4 Daniel Scott-Algara , Maryline Mancini-Bourgine , Helene Fontaine , Stanislas Pol , Marie-Louise Michel2,3,5* ´ ´ ´ ` ´ 1 Unite de Regulation des Infections Retrovirales, Institut Pasteur, Paris, France, 2 Laboratoire de pathogenese des virus de l’hepatite B, Institut Pasteur, Paris, France, ´ ´ ´ ˆ ´ ´ 3 Institut National de la Sante et de la Recherche Medicale U845, Paris, France, 4 AP-HP, Service d’hepatologie, Hopital Cochin, Universite Rene Descartes Paris V, INSERM U ´ ˆ 567, Paris, France, 5 AP-HP, Service d’hepatologie, Hopital Cochin, Paris, France Abstract Background: Improvements to the outcome of adaptive immune responses could be achieved by inducing specific natural killer (NK) cell subsets which can cooperate with dendritic cells to select efficient T cell responses. We previously reported the induction or reactivation of T cell responses in chronic hepatitis B patients vaccinated with a DNA encoding hepatitis B envelope proteins during a phase I clinical trial. Methodology/Principal Findings: In this study, we examined changes in the peripheral NK cell populations occurring during this vaccine trial using flow cytometry analysis. Despite a constant number of NK cells in the periphery, a significant increase in the CD56bright population was observed after each vaccination and during the follo