changes in whole blood gene expression in obese subjects with type 2 diabetes following bariatric surgery a pilot study全血基因表达的变化与2型糖尿病肥胖受试者减肥手术后一个试点研究.pdfVIP

Changes in Whole Blood Gene Expression in Obese Subjects with Type 2 Diabetes Following Bariatric Surgery: a Pilot Study 1,2 3,4 5 6 1,4 Stela Z. Berisha , David Serre , Philip Schauer , Sangeeta R. Kashyap *, Jonathan D. Smith * 1 Department of Cell Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, 2 Department of Biological, Geological, and Environmental Sciences, Cleveland State University, Cleveland, Ohio, United States of America, 3 Genomic Medicine Institute, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, 4 Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio, United States of America, 5 Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, 6 Department of Endocrinology, Diabetes and Metabolism, Cleveland Clinic, Cleveland, Ohio, United States of America Abstract Background: A pilot study was performed in order to investigate the effects of bariatric surgery on whole blood gene expression profiles in obese subjects with type 2 diabetes. Methodology/Principal Findings: Whole blood from eleven obese subjects with type 2 diabetes was collected in PAXgene tubes prior to and 6–12 months after bariatric surgery. Total RNA was isolated, amplified, labeled and hybridized to Illumina gene expression microarrays. Clinical and expression data were analyzed using a paired t-test, and correlations between changes in clinical trait and transcript levels were calculated. Pathways were identified using Ingenuity Pathway Analysis and DAVID gene ontology software.