corals use similar immune cells and wound-healing processes as those of higher organisms珊瑚使用类似的免疫细胞和愈合过程的高等生物.pdfVIP

Corals Use Similar Immune Cells and Wound-Healing Processes as Those of Higher Organisms 1,2 3 1 2 Caroline V. Palmer *, Nikki G. Traylor-Knowles , Bette L. Willis , John C. Bythell 1 ARC Centre of Excellence for Coral Reef Studies and School of Marine and Tropical Biology, James Cook University, Townsville, Queensland, Australia, 2 School of Biology, Newcastle University, Newcastle upon Tyne, United Kingdom, 3 Department of Biology, Boston University, Boston, Massachusetts, United States of America Abstract Sessile animals, like corals, frequently suffer physical injury from a variety of sources, thus wound-healing mechanisms that restore tissue integrity and prevent infection are vitally important for defence. Despite the ecological importance of reef- building corals, little is known about the cells and processes involved in wound healing in this group or in phylogenetically basal metazoans in general. A histological investigation into wound healing of the scleractinian coral Porites cylindrica at 0 h, 6 h, 24 h and 48 h after injury revealed differences in cellular components between injured and healthy tissues. Cell counts of the obligate endosymbiont, Symbiodinium, and melanin volume fraction analysis revealed rapid declines in both Symbiodinium abundance and tissue cross-sectional area occupied by melanin-containing granular cells after injury. Four phases of wound healing were identified, which are similar to phases described for both vertebrates and invertebrates. The four phases included (i) plug formation via the degranulation of melanin-containing granular cells; (ii) immune cell infiltration (inflammation); (iii) granular tissue formation (proliferation); and (iv) maturation.