comparative host specificity of human- and pig- associated staphylococcus aureus clonal lineages比较宿主特异性的人类,猪,金黄色葡萄球菌克隆血统有关.pdfVIP

Comparative Host Specificity of Human- and Pig- Associated Staphylococcus aureus Clonal Lineages 1 1 2 3 3 Arshnee Moodley *, Carmen Espinosa-Gongora , Søren S. Nielsen , Alex J. McCarthy , Jodi A Lindsay , Luca Guardabassi1 1 Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark, 2 Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark, 3 Department of Clinical Sciences, St George’s University of London, London, United Kingdom Abstract Bacterial adhesion is a crucial step in colonization of the skin. In this study, we investigated the differential adherence to human and pig corneocytes of six Staphylococcus aureus strains belonging to three human-associated [ST8 (CC8), ST22 (CC22) and ST36(CC30)] and two pig-associated [ST398 (CC398) and ST433(CC30)] clonal lineages, and their colonization potential in the pig host was assessed by in vivo competition experiments. Corneocytes were collected from 11 humans and 21 pigs using D-squameH adhesive discs, and bacterial adherence to corneocytes was quantified by a standardized light microscopy assay. A previously described porcine colonization model was used to assess the potential of the six strains to colonize the pig host. Three pregnant, S. aureus-free sows were inoculated intravaginally shortly before farrowing with different strain mixes [mix 1) human and porcine ST398; mix 2) human ST36 and porcine ST433; and mix 3) human ST8, ST22, ST36 and porcine ST398] and the ability of individual strains to colonize the nasal cavity of newborn piglets was evaluated for 28 days after birth by strain-specific