crustacean hyperglycemic hormone (chh) as a modulator of aggression in crustacean decapods甲壳纲动物高血糖的激素(chh)作为调制器甲壳纲动物的攻击性十足类.pdfVIP

Crustacean Hyperglycemic Hormone (cHH) as a Modulator of Aggression in Crustacean Decapods 1 2 2 3 2 Laura Aquiloni *, Piero G. Giulianini , Alessandro Mosco , Corrado Guarnaccia , Enrico Ferrero , Francesca Gherardi1 ` ` 1 Dipartimento di Biologia Evoluzionistica ‘‘Leo Pardi’’, Universita degli Studi di Firenze, Firenze, Italy, 2 Dipartimento di Scienze della Vita, Universita degli Studi di Trieste, Trieste, Italy, 3 International Centre for Genetic Engineering and Biotechnology, AREA Science Park, Trieste, Italy Abstract Biogenic amines, particularly serotonin, are recognised to play an important role in controlling the aggression of invertebrates, whereas the effect of neurohormones is still underexplored. The crustacean Hyperglycemic Hormone (cHH) is a multifunctional member of the eyestalk neuropeptide family. We expect that this neuropeptide influences aggression either directly, by controlling its expression, or indirectly, by mobilizing the energetic stores needed for the increased activity of an animal. Our study aims at testing such an influence and the possible reversion of hierarchies in the red swamp crayfish, Procambarus clarkii, as a model organism. Three types of pairs of similarly sized males were formed: (1) ‘control pairs’ (CP, n = 8): both individuals were injected with a phosphate saline solution (PBS); (2) ‘reinforced pairs’ (RP, n = 9): the alpha alone was injected with native cHH, and the beta with PBS; (3) ‘inverted pairs’ (IP, n = 9): the opposite of (2). We found that, independently of the crayfish’s prior soc