cxcl8 chemokines in teleost fish two lineages with distinct expression profiles during early phases of inflammationcxcl8趋化因子在硬骨鱼鱼两个血统与不同的表达谱在炎症的早期阶段.pdfVIP

CXCL8 Chemokines in Teleost Fish: Two Lineages with Distinct Expression Profiles during Early Phases of Inflammation 1,2. 1,3. 1 2 Lieke M. van der Aa , Magdalena Chadzinska , Edwin Tijhaar , Pierre Boudinot , B. M. Lidy Verburg-van Kemenade1* ´ 1 Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands, 2 Virologie et Immunologie Moleculaires, Institut National de la Recherche Agronomique, Jouy-en-Josas, France, 3 Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, Krakow, Poland Abstract Background: During the inflammatory process, chemokine CXCL8 plays a pivotal role in recruitment of human neutrophilic granulocytes. A diversity of sequences similar to CXCL8 was reported in fish, but their evolutionary relationships and functional homology with their human homolog remain unclear. Principal Findings: We screened fish genomes to seek for sequences related to CXCL8. A first lineage was retrieved in all teleosts, while a second CXCL8 lineage was found in zebrafish and carp only. An early inflammatory function for both lineages was indicated by several lines of evidence. The induction of carp CXCL8s, CXCb, and CXC receptor-1 and -2 was analyzed after in vitro stimulation of leukocyte subpopulations and in two in vivo inflammation models. Recombinant proteins of carp CXCL8 proteins were produced and showed significant chemotactic activity for carp leukocytes. Conclusions: While both carp CXCL8s appear to be functional homologs of