dengue virus infection-enhancing activity in serum samples with neutralizing activity as determined by using fcγr-expressing cells登革病毒血清样本infection-enhancing活动中和活动由使用fcγr-expressing细胞.pdfVIP

Dengue Virus Infection-Enhancing Activity in Serum Samples with Neutralizing Activity as Determined by Using FccR-Expressing Cells 1 1 2 1 1 Meng Ling Moi , Chang-Kweng Lim , Kaw Bing Chua , Tomohiko Takasaki , Ichiro Kurane * 1 Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan, 2 National Public Health Laboratory, Ministry of Health, Sungai Buloh, Selangor, Malaysia Abstract Background: Progress in dengue vaccine development has been hampered by limited understanding of protective immunity against dengue virus infection. Conventional neutralizing antibody titration assays that use FccR-negative cells do not consider possible infection-enhancement activity. We reasoned that as FccR-expressing cells are the major target cells of dengue virus, neutralizing antibody titration assays using FccR-expressing cells that determine the sum of neutralizing and infection-enhancing activity, may better reflect the biological properties of antibodies in vivo. Methods and Findings: We evaluated serum samples from 80 residents of a dengue endemic country, Malaysia, for neutralizing activity, and infection-enhancing activity at 1:10 serum dilution by using FccR-negative BHK cells and FccR- expressing BHK cells. The serum samples consisted of a panel of patients with acute DENV infection (31%, 25/80) and a panel of donors without acute DENV infection (69%, 55/80). A high proportion of the tested serum samples (75%, 60/80) demonstrated DENV neutralizing activity (PRNT50$10) and infection-enhancing activity. Eleven of 18 serum samples from patients with acute secondary DENV infection demonstrated neutralizing activity to the infecting serotype determined by