derivation of rhesus monkey parthenogenetic embryonic stem cells and its microrna signature推导的恒河猴孤雌生殖的胚胎干细胞及其微rna的签名.pdfVIP

Derivation of Rhesus Monkey Parthenogenetic Embryonic Stem Cells and Its MicroRNA Signature Qiang Wei1,3.*, Zhenghua Sun2,3., Xiechao He1., Tao Tan1,3, Bin Lu1,3, Xiangyu Guo1,3, Bing Su2,3*, Weizhi Ji1,3* 1 Department of Reproduction and Development, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China, 2 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China, 3 Graduate School of Chinese Academy of Sciences, Beijing, China Abstract Parthenogenetic embryonic stem cells are considered as a promising resource for regeneration medicine and powerful tools for developmental biology. A lot of studies have revealed that embryonic stem cells have distinct microRNA expression pattern and these microRNAs play important roles in self-renewal and pluripotency of embryonic stem cells. However, few studies concern about microRNA expression pattern in parthenogenetic embryonic stem cells, especially in non-human primate—the ideal model species for human, largely due to the limited rhesus monkey parthenogenetic embryonic stem cells (rpESCs) available and lack of systematic analysis of the basics of rpESCs. Here, we derived two novel rpESCs lines and characterized their microRNA signature by Solexa deep sequencing. These two novel rpESCs shared many properties with other primate ESCs, including expression of pluripotent markers, capacity to generate derivatives representative of all three germ layers in vivo and in vitro, maintaining of euploid karyotype even after long culture. Additionally, lack of some paternally expressed imprinted genes and identity of Single-nucleotide Polymorphism (SNP) compare to their oocyte donors support their parthenogenesis origin. By characterizing their microRNA signature, we identified 91 novel microRNAs